Thermo Fisher, 12489019, pcDNA™3.2-DEST Mammalian Expression Vector

pcDNA™3.
2-DEST Mammalian Expression Vector The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.
2/V5-DEST vector offers the following key features:
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination withattL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coliGateway™ CloningTo fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression inE. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors haveattR sites for recombination with anyattL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately:Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Constitutive Expression
Product Type: Mammalian Expression Vector
Quantity: 6 μg
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: pDEST, pcDNA
Cloning Method: Gateway
Product Line: Gateway™, pcDNA™
Promoter: CMV
Protein Tag: V5 Epitope Tag
Unit Size: Each