Thermo Fisher, 12535029, PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway™ Technology. The PCR Cloning System with Gateway™ Technology is designed to create an entry clone following PCR withattB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway™ Technology offers:
• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Cloning Method: Gateway™
No. of Reactions: 20 reactions
Product Line: One Shot™
Product Type: PCR Cloning System
Quantity: 20 Reactions
Vector: pDONR
Unit Size: Each