Thermo Fisher, 21566, Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate)) Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.
5.
Features of Sulfo-EGS:
•Reactive groups:sulfo-NHS ester(both ends)
•Reactive towards:amino groups (primary amines)
• Water-soluble; compare toEGS
• Membrane-impermeable, allowing for cell surface labeling
• Crosslinks formed are reversible at pH 8.
5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGSProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: No
Description: Sulfo-EGS
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 660.
45
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Sulfo-NHS Ester
Shipping Condition: Ambient
Solubility: Water
Spacer Arm Length: 16.
1 Å
Water Soluble: Yes
Chemical Reactivity: Amine-Amine
Cleavable: By Hydroxylamine
Crosslinker Type: Homobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each