Thermo Fisher, 23263, Pierce™ Colorimetric Protease Assay Kit

Pierce™ Colorimetric Protease Assay Kit The Thermo Scientific Pierce Colorimetric Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples. This kit uses fully succinylated casein as a substrate for the assay. Hydrolysis of this substrate in the presence of protease results in the release of peptide fragments with free amino-terminal groups. These peptides are reacted with trinitrobenzene sulfonic acid (TNBSA), followed by measurement of the absorbance increase that results from the formation of yellow colored TNB-peptide adducts.
Features of the Colorimetric Protease Assay Kit:
•Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
•Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
•Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
•Simple and fast—test tube and microplate protocols are completed in less than one hour
•Homogenous—addition steps only; no separation, transfer or stop steps required
•Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interestApplications:
• Assess functional integrity and overall progress of protease purification
• Quantify protease contamination in protein samples
• Characterize the activity of unusual proteases compared to trypsin
• Evaluate buffer conditions for their affects on protease function and activityAdditional Specifications:
• Substrate: succinylated casein (not available separately)
• Basis of assay: detection of primary amines resulting from proteolysis
• Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
• Measurement: absorbance at 450nm (plate reader or spectrophotometer)Assay: Protease Assay
For Use With (Application): Absorbance
For Use With (Equipment): Microplate Reader
Product Line: Pierce™
Product Type: Protein Activity Assay
Quantity: 250 Test Kit
Substrate: Casein
Substrate Properties: Protein-Based Substrate
Substrate Type: Protease Substrate
Sufficient For: 250 Microplate Assays
Target: Proteases
Detection Method: Colorimetric
Immunoassay Kit Format: 96-well Plate
Unit Size: Each