Thermo Fisher, 34035, NBT Substrate Powder (nitro-blue tetrazolium chloride)

NBT Substrate Powder (nitro-blue tetrazolium chloride) Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).
Features:
•AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
•Chromogenic—no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
•Package options—choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressorHighlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042):
• Best for immunoblotting applications
• Ready-to-use single component
• Sensitive with low background and minimial assay-to-assay variabilityHighlights of Pierce NBT/BCIP plus Suppressor 1-Step Solution (Part No. 34070):
• Best for immunohistochemistry applications
• Ready-to-use single component
• Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphataseDescription: Pierce NBT Substrate
Reactivity: Alkaline Phosphatase
Detection Method: Colorimetric
Form: Powder
Membrane Compatibility: Nitrocellulose, PVDF
Product Type: Substrate
Quantity: 1 g
Substrate: Alkaline Phosphatase
Unit Size: Each