Thermo Fisher, 34096, SuperSignal™ West Femto Maximum Sensitivity Substrate

SuperSignal™ West Femto Maximum Sensitivity Substrate Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.
SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
•Sensitivity:low-femtogram (mid-zeptomole)
•Stability:8-hr working solution stability, 1-year kit stability at 4°C
•Compatibility:nitrocellulose and PVDF membranes
•Signal duration:up to 8 hours
•Recommended primary antibody concentration:1:5,000–1:100,000 dilution (10–200 ng/mL)
•Recommended secondary antibody concentration:1:100,000–1:500,000 dilution (2–10 ng/mL)Compare and learn more about our western blot chemiluminescent substrates ›View all HRP substrates available ›SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.
Conjugate: HRP
Kit Contents: Stable Peroxide Buffer (100 mL), Luminol/Enhancer (100 mL)
Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Sensitivity: Low femtogram-level
Signal Duration: 6 to 8 hr.
Sufficient For: 25 mini-gel blots, 2000 sq. cm of membrane
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Product Line: SuperSignal™
Product Type: Substrate
Quantity: 200 mL
Substrate: Horseradish Peroxidase
Unit Size: Each