Thermo Fisher, 4485712, TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe

Sufficient For: 250 Reactions The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The assay consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.
Benefits of this assay include:
• Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assaysSave time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.
Accession Number: NM_002046.
3
Format: Tube
Gene Symbol: GAPDH
Green Features: Sustainable packaging
Internal Probe Modification: QSY™ Non-Fluorescent Quencher (3')
No. of Reactions: 250 Reactions
Product Line: TaqMan™
Product Type: Gapdh Assay
Quantity: 250 reactions
Species: Human
Sufficient For: 250 Reactions
Target: Glyceraldehyde-3-phosphate dehydrogenase
Detection Method: Primer-probe
For Use With (Application): Gene Expression
Label or Dye: JUN
PCR Method: qPCR
Unit Size: Each