Thermo Fisher, 75706, beta-Galactosidase Assay Stop Solution

beta-Galactosidase Assay Stop Solution The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):Choose stand-alone assay reagent or complete kit for cell lysis and detectionColorimetric assay easily measured in a plate reader or spectrophotometer (405 nm)Adaptable to 96-well microplates or tube assay formatsPerform homogeneous screening assays or measure activity secondarily after cell lysisAlso compatiible with prepared lysates from other species such as bacteriaDetection Method: Reporter Enzyme
Dye Type: Enzyme Labeled
Format: 96-well Plate
Quantity: 25 mL
Substrate: Proprietary
Substrate Type: Beta-Gal Substrate
Target: Beta-Galactosidase
For Use With (Application): Absorbance
For Use With (Equipment): Microplate Reader
Product Line: Pierce
Product Type: β-Galactosidase Assay Stop Solution
Unit Size: Each