Thermo Fisher, A15145, LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit, rabbit

LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit, rabbit This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4894:The LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor-gamma (PPAR gamma). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and human PPAR gamma ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:When running the LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR gamma-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR gamma, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR gamma is detected by a test compound's ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.
Assay Category: Biochemical Assay
Color: Green
Detection Method: Fluorescence
Druggable Target: Nuclear Receptors
For Use With (Application): Nuclear Receptor Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Ligand: Pan-PPAR
No. of Assays: 400 x 40 μL Assays
Packaging: 384-well Plate
Product Type: TR-FRET PPAR Gamma Competitive Binding Assay Kit
Quantity: 400 x 40 μL assays
Readout: TR-FRET (Binding)
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each