Thermo Fisher, A34717, TaqMan™ Advanced miRNA Human Serum/Plasma Card

TaqMan™ Advanced miRNA Human Serum/Plasma Card The Applied Biosystems TaqMan Advanced Serum/Plasma Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains one card for profiling of up to 188 unique miRNAs in one serum/plasma sample, and includes endogenous and exogenous miRNA controls for normalization of data results.
Features of the TaqMan Advanced Serum/Plasma Card include:
•Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
•Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
•Updated content—assay content updated for mirBase v22
•Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
•Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA familiesProduct detailsNumber of cards:1Number of unique miRNAs (not including exogenous controls):188Exogenous controls:cel-miR-39-3p, ath-miR159aEndogenous control:hsa-miR-16-5pSamples per card:1Compatible with the following Applied Biosystems qPCR instruments:Viia7, QuantStudio 7 Flex, and QuantStudio 12k FlexRecommended master mix:TaqMan Fast Advanced Master MixNotes
• TaqMan Advanced miRNA plates require the use of theTaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For more information on the availability and pricing for Advanced miRNA assays in pre-spotted 384 well plates, please emailspecialty_cards@thermofisher.
com.
Format: 384-well microfluidic card
Product Line: TaqMan™
Product Type: Advanced miRNA Human Serum/Plasma Card
Quantity: 384 reactions
Research Category: miRNA
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Species: Human
Volume: 2 μL
Detection Method: Primer-probe
For Use With (Application): miRNA analysis
GC-Rich PCR Performance: High
Label or Dye: 2 Color
PCR Method: qPCR
Reaction Speed: Fast or Standard
Unit Size: Each