Thermo Fisher, A38556, SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignal West Atto substrate provides the highest-level sensitivity and better signal-to–noise ratios compared to other commercially available high-performance horseradish peroxidase (HRP) substrates.
SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate characteristics include:Sensitivity: low-femtogram to high-attogramStability: 48 hours for working solution, one year for kit at 4°CCompatibility: nitrocellulose and PVDF membranesSignal duration: up to six hoursRecommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.
2–1.
0 μg/mL)Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)Compare and learn more about our western blot chemiluminescent substrates ›View all HRP substrates available ›SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is designed for superior sensitivity and signal-to–noise ratios for very low abundant targets or precious samples in western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Atto substrate enables detection of target proteins in amounts that are too small to be seen with typical high performance ECL substrates.
Sensitivity: Low-femtogram to high-attogram
Shipping Condition: Wet Ice
Sufficient For: 25 Mini-Gel Blots, 2000 sq. cm of Membrane
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Product Line: SuperSignal™
Product Type: Substrate
Quantity: 200 mL
Substrate: Horseradish Peroxidase
Unit Size: Each