Thermo Fisher, A39242CN, Expi293F™ Inducible GnTI- Cells, China

Expi293F™ Inducible GnTI- Cells, China The Expi293F Inducible GnTI- cell line is derived from engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The cell line was established by the selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.
Features of Expi293F Inducible GnTI- cells include:
• Suspension, high-density culture in Expi293 Expression Medium
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/LFor inducible expression of your protein of interest,pcDNA5/TO mammalian expression vectoror an equivalent inducible vector is required for tetracycline-regulated gene expression.
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.
Product Line: Expi293™
Quantity: 1 vial
Shipping Condition: Dry Ice
Unit Size: Each