Thermo Fisher, C202003, One Shot™ INVαF' Chemically Competent E. coli

One Shot™ INVαF' Chemically Competent E. coli One Shot INVαF´ Chemically CompetentE. coliare designed to stably replicate high-copy number plasmids. They allow for the preparation of high quality plasmid DNA with less carry over contaminants as compared to other popular strains such as JM109 and can achieve a transformation efficiency of >1 x 108cfu/μg supercoiled plasmid.
INVαF´ cells carry the F´ episome and so can be utilized to produce single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. This strain is related to DH strains and features similar genetic traits and benefits. ThelacZΔM15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and can be used for blue-white screening of colonies on bacterial plates containing Bluo-Gal or X-Gal. In addition to supporting blue/white screening,recA1 andendA1 mutations increase insert stability and improve the quality of plasmid DNA.
One Shot INVαF´ Chemically CompetentE. colicells offer:
• Transformation efficiencies of >1 x 108cfu/μg
• Stability and exceptional quality of high-copy number plasmid DNAb
• Blue-white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA1)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of ssDNA from vectors carrying the f1 origin of replication (F´)Note:INVαF´ cells do not require IPTG to induce expression from thelacpromoter.
Convenient and efficient formatOne Shot INVαF´ Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one complete transformation, reducing freeze-thaw cycles and limiting money wasted on unused cells.
GenotypeF´endA1recA1hsdR17 (rK–, mK+)supE44thi-1gyrA96relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 λ–Genetic marker descriptionsFind the strain and format that fit your needsA variety of DH strains are available inchemically competentandelectrocompetent cellformats.
We offer other strains for ssDNA production, such asTOP10F´or electrocompetentDH12S.
MultiShot formatsare available for high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (hsd)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: Yes
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: High Copy Plasmid
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL/tube
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each