Thermo Fisher, C404003, One Shot™ TOP10 Chemically Competent E. coli

21 x 50 μL One Shot TOP10 Chemically CompetentE. coliare ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109cfu/μg plasmid DNA. These cells allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. TOP10E. colicells are genetically similar to the DH10B strain and have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress. One Shot TOP10 cells:
• Maximize cloning efficiency in a single-tube format
• Provide enhanced genomic DNA cloning capabilitiesTOP10 Chemically CompetentE. colicells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, TOP10 has thelacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. The inclusion ofrecA1 andendA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps. One Shot TOP10 Chemically CompetentE. colicells offer:
• Transformation efficiencies of >1 x 109cfu/μg
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNA
• Expression from thelacpromoter without IPTGEasy-to-use One Shot formatTOP10 Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format accomodates allows all steps of the transformation protocol, up to plating, to take place in the same tube, helping save time and prevent contamination. GenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalK λ–rpsL(StrR)endA1nupGFind the strain and format that fit your needsWe offer other DH strains inchemically competentandelectrocompetent cellformats. The TOP10 strain is available in severalMultiShot formatsfor high throughput applications. Explorebacterial growthmedia formats. Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: High Copy Plasmid
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each