Thermo Fisher, C640003, MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp™ Cells MegaX DH10B T1RElectrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010cfu/μg of pUC DNA, making MegaX DH10B T1Rcells ideal for highly demanding cloning or cDNA and gDNA library construction applications. The DH10B T1Rstrain has all the benefits of DH10B genotype, with the addition of thetonA (also known asfhuA) genotype that confers resistance to T1 and T5 phage infection. The inclusion of thetonA genotype offers added security to protect valuable clones and libraries. MegaX DH10B T1Rcells carry mutations in the methylation-dependentE. coliK12 restriction system (mcrA,mcrBC, andmrr) allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has thelacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, theendA1 mutation makes DH10B T1Ran excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification. MegaX DH10B T1RElectrocomp Cells offer:
• Highest transformation efficiency of >3 x 1010cfu/μg
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
•lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
•mcrA genotypic marker and themcrBC,mrrdeletion allows for cloning DNA that contains methylcytosine and methyladenine
•endA1 mutation enables increased plasmid yield and quantity
•recA1 for reduced occurrence of nonspecific recombination in cloned DNANote:A high-voltage electroporation apparatus is required. GenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1endA1araD139 Δ(ara-leu)7697galUgalK λrpsL(StrR)nupGtonAFind the strain and format that fits your needsWe offer other DH strains inchemically competentandelectrocompetent cellformats to meet your specific needs. Electroporation cuvettes are available in0. 1 cm,0. 2 cm, and0. 4 cmgaps. Strains are available in severalMultiShot formats for high throughput applications. Explorebacterial growthmedia formats. Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >100 kb
Preparing Unmethylated DNA: No
Product Line: Electrocomp™
Product Type: Electrocompetent Cells
Quantity: 5 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>3 x 1010cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each