Thermo Fisher, C6481201, MultiShot™ FlexPlate DH10B T1R Competent Cells

MultiShot™ FlexPlate DH10B T1R Competent Cells MultiShot FlexPlate DH10B T1Rchemically competentE. colicells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be conveniently divided into 8-well segments for flexibility in transformation throughput.
All of our T1Rstrains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyseE. colihosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.
Diverse DH10B T1Rcloning capabilitiesMultiShot FlexPlate DH10B T1R competent cells may be used in a variety of cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 108cfu/μg with control plasmid DNA. The cells are ideal for cloning large plasmids and BACs and are suitable for cloning DNA that contains methylcytosine and methyladenine (i.
e., genomic DNA).
MultiShot FlexPlate DH10B T1R Competent Cell features
•mcrA,mcrBC,mrr, andhsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
•endA1 for increased plasmid yield and quantity
•recA1 for reduced occurrence of non-specific recombination in cloned DNA
•lacZΔM15 for blue/white color screening of recombinant clonesIncrease transformation productivity with MultiShot FlexWell formatThe FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible 96-well plate may be divided into 8-wellsstrips
• Competent cells come pre-aliquoted in 96-well plates and ready for transformationGenotypeF-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15lacX74recA1endA1araD139 Δ(ara-leu)7697galUgalKrpsLnupG λ-tonAFind the strain and format that you needWe offer manyE. colistrains and formats ofchemically competentandelectrocompetent cellsto meet your specific transformation needs. Choose from ourMultiShot formatted comp cellsfor high-throughput options oremail usfor custom formatting information.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes
Contains F' Episome: No
High-throughput Compatibility: High
Plasmid: May be used for plasmids >100 kb
Preparing Unmethylated DNA: No
Product Line: Multishot™
Product Type: Chemically Competent Cells
Quantity: 1 x 96 well plate (20 μl/well)
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Format: 96-well Plate
Species: E. coli (K12)
Unit Size: Each