Thermo Fisher, CS10201, ChargeSwitch™ NoSpin Plasmid Micro Kit

ChargeSwitch™ NoSpin Plasmid Micro Kit The ChargeSwitch NoSpin Plasmid Micro Kit provides centrifuge-free microprep plasmid purification that is not only ideal for automation but is adaptable to the KingFisher Flex Purification System. The novel NoSpin reagent causes bacterial cell flocculation and allows cells to be pelleted without the need for automation bottlenecks like centrifugation. This kit includes all the necessary reagents for 0.
5–1 mL overnight culture samples with a typical yield of up to 5 μg and requires the use of a magnetic accessory or automation system such as the KingFisher Flex system.
• Provides centrifuge-free purification
• Avoids introduction of enzymatic contaminants for better results
• Optimized for automation
• Ideal for use on the KingFisher Flex systemPerformance improvementsThis ChargeSwitch NoSpin Plasmid Micro Kit workflow has been adapted to the KingFisher Flex system to allow for purification of 96 samples in about half the time it takes to perform manually. The use of this automated platform results in better performance of the workflow for increased yield (3-fold), purity, and integrity (supercoiling increased from 50% to 90%) (see figures).
Expected Yield (manual):up to 5 μgExpected Yield (automated):up to 10 μgPlasmid DNA size:any size plasmid or cosmidNumber of steps (manual):30Number of 96 deep well plates:7Total time (manual):90 minAutomated time (hands on + script):14 min + 30 min (44 min)Wash steps:2Elution volume (μL):100Final Product Type: Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: High-throughput Compatible (KingFisher Flex)
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Line: ChargeSwitch™
Product Type: NoSpin Plasmid Micro Kit
Quantity: 96 Preps
Sample Type: Bacterial Culture
Shipping Condition: Room Temperature
Target: Plasmid DNA
Format: Kit
Isolation Technology: ChargeSwitch Chemistry
Unit Size: Each