Thermo Fisher, E3599, Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO

Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO The cell-impermeant viability indicator ethdium homodimer-2 (EthD-2) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ∼535/624).
Note: A protocol is not included with this classic reagent as they are readily available in the literature. The reagent is provided already dissolved at 1 mM in DMSO. A typical protocol for staining dead cells is to prepare a working solution at 0.
1 to 10 μM in a physiological buffer and incubate with cells for 30-45 minutes at room temperature before analyzing on a fluorescent microscope, flow cytometer, or plate reader.
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Color: Orange
Description: Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO
Detection Method: Fluorescence
Dye Type: Cell-Impermeant
Emission: 624 nm
Excitation Wavelength Range: 535 nm
For Use With (Application): Cell staining assays
For Use With (Equipment): Fluorescence Microscope
Quantity: 200 μL
Shipping Condition: Room Temperature
Label Type: Fluorescent Dye
Product Type: Viability Indicator
SubCellular Localization: Nucleic Acids, Nucleus
Unit Size: Each