Thermo Fisher, K250020SC, pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only)

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only) TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. TOPO™ TA Cloning™ Kits use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. The newest TOPO™ TA Cloning™ Kit comes with a Gateway™ (GW) entry vector, pCR™8/GW/TOPO™ (Figure 1). This vector has:
• 3´-T overhangs for direct ligation ofTaq-amplified PCR products
•attsites for rapid recombination into a variety of Gateway™ destination vectors
• Novel GW primer sites, within theattregions, located less than 55 base pairs from the PCR product insertion site for convenient sequencing
• Spectinomycin resistance gene for robust selection inE. coli
•EcoR I sites flanking the PCR product insertion site for easy excision of insertsSelected TOPO™ TA Cloning™ Kits for entry into the Gateway™ Technology are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
Cloning Method: TOPO™ with Gateway™
No. of Reactions: 20 reactions
Product Line: One Shot™
Product Type: TA Cloning Kit
Quantity: 20 Reactions
Shipping Condition: Dry Ice
Vector: pCR
Unit Size: Each