Thermo Fisher, K413001, DES™-Inducible/Secreted Kit with pCoHygro

DES™-Inducible/Secreted Kit with pCoHygro The DES™-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:
• Small size (3.
6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resinTo facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
Inducing Agent: Copper Sulfate
Product Type: Inducible/Secreted Kit
Quantity: 1 kit
Vector: pMT
Cloning Method: Restriction Enzyme/MCS
Product Line: DES™
Promoter: MT
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each