Thermo Fisher, K490040, pcDNA™3.1 Directional TOPO™ Expression Kit

40 Reactions The pcDNA™3.
1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.
1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientationIn addition, pcDNA3.
1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Constitutive Expression
Product Type: TOPO Expression Kit
Quantity: 40 Reactions
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: Directional TOPO Vectors, pcDNA
Cloning Method: Directional TOPO
Product Line: TOPO™, pcDNA™
Promoter: CMV
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each