Thermo Fisher, K495510, pLenti6/V5 Directional TOPO™ Cloning Kit

pLenti6/V5 Directional TOPO™ Cloning Kit The pLenti6⁄V5 Directional TOPO™ Cloning Kit contains the TOPO™-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.
Advantages
• High efficiency and rapid cloning
• Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the systemKey Features
• Directional TOPO™ Cloning site for rapid and efficient directional cloning of blunt-end PCR products
• Rouse Sarcoma Virus (RSV) enhancer⁄promoter for Tat-independent production of viral mRNA in the producer cell line
• Modified HIV-1 5’ and 3’ Long Terminal Repeats (LTR) for viral packaging and reverse transcription
• HIV-1 psi (ψ) packaging sequence for viral packaging
• HIV Rev response element (RRE) for Rev-dependent nuclear export of unspliced viral mRNA
• (CMV) immediate early promoter for high-level constitutive expression of the gene of interest in mammalian cells
• C-terminal V5 epitope for detection of the recombinant protein
• Blasticidin (bsd) resistance gene for selection inE. coliand mammalian cells
• Ampicillin resistance gene for selection inE. coli
• pUC origin for high-copy replication and maintenance of the plasmid inE. coliKit includespLenti6⁄V5-D- TOPO™ ReagentsOne Shot™ Stbl3™ Chemically CompetentE. coliFor research use only. Not intended for any therapeutic or diagnostic use.
Cloning Method: Directional TOPO™
Constitutive or Inducible System: Constitutive
Delivery Type: Lentiviral
For Use With (Application): Viral Expression
No. of Reactions: 20 reactions
Product Line: TOPO™, ViraPower™
Product Type: TOPO Cloning Kit
Promoter: CMV
Protein Tag: V5 Epitope Tag
Quantity: 20 Reactions
Selection Agent (Eukaryotic): Blasticidin
Vector: pLenti, Directional TOPO Vectors
Unit Size: Each