Thermo Fisher, K59120, pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli The pENTR™ 5’-TOPO™ TA Cloning™ Kit combines Invitrogen’s TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway™ entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO™ vector to a suitable MultiSite Gateway™ destination vector in a MultiSite Gateway™ LR recombination reaction with other suitable entry clone(s) to generate an expression construct.
This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway™ Vector Kit, or MultiSite Gateway™ Three-Fragment Vector Construction Kit.
Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cellsKey Features
• Rapid PCR cloning with TOPO™ cloning technology for cloning the 5’ element
• attL4 & attR1 sites for compatibility with MultiSite Gateway™ LR reaction for increased flexibilityKit Includes
• pENTR™ 5´-TOPO™ TA Cloning™ Kit
• One Shot™ TOP10 Chemically Competent E. coli (C4040-03)Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway™ Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (A11146)
• MultiSite Gateway™ Three-Fragment Vector Construction Kit (12537-023)For research use only. Not intended for any therapeutic or diagnostic use.
Product Type: TA Cloning Kit
Quantity: 20 reactions
Vector: pENTR, TOPO-TA Cloning Vectors
Cloning Method: TOPO-TA
Product Line: One Shot™
Unit Size: Each