Thermo Fisher, K990060, Expressway™Lumio™ Expression and Detection System, without vector

Expressway™Lumio™ Expression and Detection System, without vector The Expressway™ Lumio™ Expression and Detection System takes advantage of the Lumio™ recognition sequence, a small, six amino acid sequence (Cys-Cys-Pro-Gly-Cys-Cys). The Lumio™ detection reagent binds the recognition sequence with high specificity and affinity, resulting in a bright fluorescent signal for real-time protein production analysis and immediate in-gel protein detection. In addition, Expressway’s specializedE. colilysate, derived from aslyD mutant, eliminates nonspecific binding of the Lumio™ Green Detection Reagent to the endogenous SlyD protein and provides optimal background for detection of recombinant proteins (Figure 1). The Lumio™ Green Detection Kit is included in the system for real-time detection of protein synthesis as well as easy in-gel protein detection. The Lumio™ vectors (Figures 2 and 3) also feature:
•attR sites for efficient recombination with anyattL-flanked Gateway™ entry vector
•N-terminal Lumio™ tag (pEXP3-DEST vector) with TEV cleavage site for efficient removal of the Lumio™ sequence following purification
•C-terminal Lumio™ tag (pEXP4-DEST vector) for easy, and immediate in-gel detection
•T7 promoter, ribosome binding site, and T7 terminator optimally spaced for cell-free protein expressionDetection Location: In-Gel Detection
Expressed Protein Type: Water Soluble Protein
Expression Mechanism: Cell-Free Expression
Expression System: Cell-Free
Product Type: Lumio Expression and Detection System
Quantity: 1 kit
Product Line: Expressway™, Lumio™
Unit Size: Each