Thermo Fisher, L32474, Dolichos Biflorus (Horse Gram) Agglutinin (DBA), fluorescein (FITC)

Dolichos Biflorus (Horse Gram) Agglutinin (DBA), fluorescein (FITC) Dolichos Biflorus (Horse Gram) Agglutinin (DBA), fluorescein (FITC), is a bright-green DBA-fluorophor conjugate. DBA has been used to establish secretor status in blood-group-A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts. It has also been used as a marker of cell surface change in murine embryonic stem cells during early differentiation. View complete list of fluorescent dye-conjugated lectins ›
• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: N-acetylgalactosamine
• Inhibiting/eluting sugar: 200 mM N-acetylgalactosamine
• Blood group specificity: A1 >>A2
• Unconjugated lectin molecular weight: 111 KDa
• Immunofluorescence working concentration: 5–20 μg/mLDBA-FITC is provided at a 2 mg/mL concentration in 10 mM HEPES, 0. 15 M NaCl, pH 7. 5, 0. 08% sodium azide, 0. 1 mM Ca2+. If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use. Protein Family: Lectins
Quantity: 2 mg
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Source: Horse gram
Conjugate: FITC
Form: Lyophilized
Unit Size: Each