Thermo Fisher, T1028, Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

10,000 Assays The Gal-Screen™ assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.
Ideal for ScreeningThis homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen™ system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.
For Research Use Only. Not for use in diagnostics procedures.
Assay: β-Gal (lacZ) Assay
Compatible Cells: Mammalian Cells
Detection Method: Chemiluminescence
For Use With (Application): Enzyme Assay
For Use With (Equipment): Luminometer (Microplate)
Form: Liquid
High-throughput Compatibility: High-throughput Compatible
Label or Dye: Unconjugated
Product Line: Gal-Screen™, NovaBright™
Product Type: β-Galactosidase Reporter Gene Assay System
Quantity: 10,000 Assays
Readout: End Point
Sample Type: Cell Cultures
Substrate: Galacton-Star™
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Unit Size: 10,000 assays