Thermo Fisher, V35005, Vybrant™ DyeCycle™ Orange Stain

Vybrant™ DyeCycle™ Orange Stain Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.
• Precise—accurate cell cycle analysis in living cells
• Safe—low cytotoxicity for cell sorting and additional live cell experiments
• Simple, robust staining protocolView aselection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.
PreciseSuccessful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).
Low CytotoxicityUnlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
Simple, Robust Staining ProtocolFor cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106cells/mL. To each tube add 2 μL of Vybrant™ DyeCycle™ Orange Stain. Final stain concentration is 10 μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
Concentration: 5 mM
Detection Method: Fluorescence
Dye Type: Vybrant™ DyeCycle™ Orange
Form: Solution
Format: Tube(s)
Quantity: 400 μL
Shipping Condition: Room Temperature
Solubility: Water
Sub Cellular Localization: Nucleic Acids
Emission: 532/488
For Use With (Equipment): Flow Cytometer
Product Line: DyeCycle
Product Type: Stain
Unit Size: 400 µL