Thermo Fisher, V6694, Vybrant™ Phagocytosis Assay Kit

Vybrant™ Phagocytosis Assay Kit The Vybrant™ Phagocytosis Assay Kit provides a way for researchers to observe and quantitate the process of phagocytosis in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle—in this case killedE. coli(K-12 strain) cells that have been labeled with the fluorescent dye fluorescein. In addition to the lyophilizedE. coliBioParticles™ component, the kit contains a trypan blue solution (to quench the fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader. The methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines.
Vybrant™ Phagocytosis Assay Kit Specifications:
• Particles are fluorescein-labeledE. coli(K-12 strain)
• Kit supplies sufficient reagents for ∼250 tests using 96-well plates
• Fluorescence is measured using a microplate reader (Ex/Em ∼480/520 nm)Find More BioParticles™ ProductsWe offer a large range of dye-labeled and unlabeledE. coli(K-12 strain),S. aureus(Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ products. Find out about these products and their applications by reviewingProbes for Following Receptor Binding and Phagocytosis—Section 16.
1in the Molecular Probes™ Handbook.
For pH-sensitive endocytosis assays, see ourpHrodo™ BioParticles™ conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Detection Method: Fluorescence
Dye Type: Classic Dyes
Format: 96-well plate
Quantity: 1 kit
Shipping Condition: Room Temperature
Species: E. coli
For Use With (Equipment): Microplate Reader, Microplate Reader
Product Line: Vybrant
Product Type: Phagocytosis Assay Kit
Unit Size: 1 kit