Waters, 186002009, Atlantis Silica HILIC Column, 100Å, 3 µm, 2.1 mm X 30 mm, 1/pk

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

Specifications
Chemistry: HILIC
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Silica
pH Range Min: 1 pH
pH Range Max: 5 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: High
Particle Shape: Spherical
Particle Size: 3 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 330
System: HPLC
Particle Technology: Silica
USP Classification: L3
Inner Diameter: 2.1 mm
Length: 30 mm
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
Atlantis Silica HILIC Column, 100Å, 3 µm, 2.1 mm X 30 mm, 1/pk Atlantis HILIC Silica columns are employed in conjunction with high organic mobile phases (>80% acetonitrile) to retain analytes that are simply too polar for reverse-phase chromatography to retain. In comparison to typical chromatographic reversed-phase techniques, HILIC separation processes enable orthogonal analyte selectivity. Using Hydrophilic Interaction Chromatography, the Atlantis Silica HILIC Column can easily separate and retain extremely polar, water-soluble basic chemical molecules. Peptides, metabolites, and actives are examples of these molecules. HILIC has increased LC/ESI-MS performance and direct compatibility with SPE solvents, in addition to retaining extremely polar molecules. It also provides complementing selectivity when compared to reversed-phase HPLC. Reversed-phase chromatography has a tough time retaining and separating very basic polar analytes. This is because they are hydrophilic and have a net positive charge at acidic pH. The Atlantis Silica HILIC Column is the appropriate lab equipment for use in metabolism, drug discovery, and combinatorial chemistry since HILIC solves these difficult separation challenges. You can maintain highly polar basic analytes with volatile mobile phases that are suited for compound ionization by ESI-MS using the Atlantis Silica HILIC Column. This means you'll receive results with higher sensitivity and lower detection limitations. This is particularly important in drug metabolism research because metabolites are generally more polar than their parent chemicals and are present in much lower concentrations. We recommend that you buy lab equipment from Waters directly to ensure that you get genuine equipment, and it enables you to evaluate the newest technology trends and available equipment for all of your lab needs from our extensive catalog. Our website also provides you with an opportunity to shop for lab equipment as needed. You should also look into Atlantis Silica HILIC VanGuard Cartridge, 100Å, 3 µm, 2.1 mm X 5 mm, 3/pk; Atlantis HILIC VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 2.1 mm and 3.0 mm I.D. Atlantis HILIC analytical columns containing 3 µm sorbent particles.

FAQ
What Are The Best Recommended Injection Solvents For HILIC? For usage with the Atlantis Silica HILIC Column, 100% organic solvents are advised. Acetonitrile, isopropanol, and methanol are examples of weak HILIC solvents. Water and dimethylsulfoxide (DMSO) should either be avoided or utilized in extremely small amounts if absolutely necessary to avoid undesirable peak shapes. Reversed-phase solid-phase extraction can be used to substitute water/DMSO with acetonitrile.