Waters, 186003027, XBridge BEH C18 Column, 130Å, 3.5 µm, 3 mm X 100 mm, 1/pk

As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 3 mm
Length: 100 mm
Carbon Load: 18 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH C18 Column, 130Å, 3.5 µm, 3 mm X 100 mm, 1/pk Get unmatched column lifetime and the flexibility to work over an unparalleled range of mobile phase pH and temperature with the XBridge BEH C18 Columns. Utilizing advanced end-capping and trifunctional bonding, the columns offer outstanding performance, reproducibility, and column lifetime across the entire range of pH. Low pH stability in the XBridge BEH C18 Columns is achieved by trifunctional bonding and end-capping which slow the rate of hydrolysis. When compared to columns packed with phases prepared using monofunctional silanes, the XBridge BEH C18 Columns exhibit little retention loss (greater hydrolytic stability) even under accelerated low-pH test conditions. Avoid peak shape degradation with the XBridge BEH C18 Columns. Chemical stability is demonstrated in the accelerated high-pH lifetime of the XBridge BEH C18 Columns as the XBridge particles use ethylene bridges within the silica matrix which resist particle dissolution. You may also be interested in other lab equipment such as the XBridge BEH C18 Column 130Å, 3.5 µm, 2.1 mm X 50 mm, 1/pk , similar to the product listed here with the same particle and pore size, but with a smaller internal diameter (I.D) and length. See the complete available range of Waters Xbridge BEH C18 Columns, shop for lab equipment , and browse our selection of exceptional products.

FAQ
What Is A C8 Column In HPLC? C8 column is a type of column used in the reversed-phase chromatography. While a hydrophobic stationary phase is used in the reversed-phase chromatography generally,
the solid support stationary phase or the column contains modified silica. This makes the silica a hydrophilic stationary phase. In addition to this, it contains alkyl groups, covalently bonded to silica, creating a hydrophobic silica stationary phase. Together with this, the mobile phase of the reverse-phase chromatography is aqueous. Therefore, this stationary phase has a high affinity to nonpolar compounds in the sample.

The C8 columns contain octyl carbon chains with eight carbons as the alkyl groups for the modification of the silica column. These carbon chains are comparatively short which reduces the hydrophobicity and the density of the stationary phase. This makes the retention time of the nonpolar compounds in the C8 column low, giving a lesser degree of separation.