Waters, 186003031, XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 50 mm, 1/pk

As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 50 mm
Carbon Load: 18 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 50 mm, 1/pk Combine cutting-edge bonding, end-capping, and particle synthesis with Waters’ signature manufacturing and quality in the XBridge BEH C18 Columns. These are developed to offer solutions to challenging chromatographic separations, keeping them up to the task of HPLC methods developments. The flexibility offered by these columns and XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk makes them useful for a wide range of separations while being familiar to separation scientists seeking method development flexibility. With the use of trifunctional bonding and innovative end-capping, you can achieve excellent reproducibility, performance, and column lifetime across the pH range 1-12. This unique combination of technologies and lab equipment is able to slow hydrolysis, improving the low-pH stability of bonded phases. When tested under accelerated low-pH test conditions, XBridge C18 columns show low retention loss and greater hydrolytic stability as compared to columns packed with phases prepared using monofunctional silanes. In accelerated high pH lifetime, chemical stability is achieved by incorporating ethylene bridges into the silica matrix, which resists particle dissolution. With BEH Technology particles working alongside the novel bonding and end-capping processes of XBridge BEH C18 columns, pH switching is possible without a performance decrease. This means a single XBridge column can be used to run under both low and high pH conditions, eliminating the time required for column changes and the necessity of multiple columns on hand for separate pH. Continuing Waters’ trend of being the industry benchmark for reproducibility, XBridge BEH C18 columns provide consistent results, assuring method development scientists that their separations can be created year after year. This reproducibility is all possible with no negative impact on column performance or column lifetime, even under demanding LC operating conditions.

FAQ
Can Phosphate Buffers Be Used With XBridge BEH C18 Columns? Phosphate buffers offer unique selectivity, excellent UV transparency, and good buffering capacity at a range of pKa values, but due to the aggressive nature of phosphate, silica-based columns often see reduced lifetime when they are being used. The XBridge columns allow for excellent performance using phosphate buffers across the entire pH range. This is possible because of improved chemical resistance of BEH Technology particles. Chromatographers can now take full advantage of phosphate buffers without sacrificing the quality of their separations.