Waters, 186003837, AccQ•Tag Ultra C18, 1.7 µm, 2.1 x 100 mm Column, 1/pK

The AccQ•Tag Ultra Columns are specifically designed for reversed-phase separation of amino acids derivatized with AccQ•Tag Ultra reagent. Each column is QC tested using Waters Amino Acid standard to ensure repeatable performance for your most demanding amino acid analysis. The 1.7 µm, 2.1 x 100 mm AccQ•Tag Ultra Column is used in Waters amino acid total system solution.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Amino Acids
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 18 %
eCord: Yes
UNSPSC: 41115709
Application: Amino Acid
Brand: AccQ-Tag Ultra
Product Type: Columns
Units per Package: 1 pk

Additional Information
AccQ-Tag Ultra RP Column 130Å 1.7 µm, 2.1 mm x 100 mm, 1/pk Accomplish accurate, high-sensitivity UPLC analyses with the AccQ-Tag Ultra RP Column, an integral component of Waters’ UPLC Amino Acid Analysis Solution. The success of this lab equipment is based on the AccQ-Tag Ultra Chemistry, a versatile solution for amino acid analysis that leads the industry. This method provides superb compositional analysis when it comes to hydrolyzed peptides and proteins. ACQUITY UPLC technology uses stationary phase particles that measure 1.7 µm in diameter to offer improved sensitivity, resolution, and speed. By combining these benefits with the ACQUITY UPLC system for separation technology, scientists are able to perform protein characterization, cell culture monitoring, and nutritional analysis in less time than other conventional methods. Find success in derivatizing amino acids, separating the derivatives with reversed-phase UPLC, and quantitating the derivatives based on UV absorbance or fluorescence intensity with the AccQ-Tag Ultra RP column. The AccQ-Tag Ultra Derivatization Kit is a pre-columnization derivatization technique for amino acids that is used in combination with the UPLC AAA Solution. The combination of these technologies enables scientists to easily obtain accurate results and achieve exceptional chromatographic resolution. Experience unparalleled quantitative results with the AccQ-Tag Ultra, as it utilizes the AccQ-Fluor Reagent in order to quickly and easily derivatize both primary and secondary amino acids using a single step. This AccQ-Tag Ultra RP column is optimized when used in conjunction with the full AccQ-Tag Ultra Chemistry package, including fully-tested reagents, columns, and eluents. All derivatizing reagents are developed specifically for amino acid analysis. The complete application solution is recommended when using the AccQ-Tag Ultra column. Use of the volume without the rest of the application solution is not supported and may lead to significant risks of inaccurate qualitative and quantitative analysis.

FAQ
What Is Amino Acid Analysis? Amino acid analysis is a technique used to determine the amino acid content of samples that contain amino acids, peptides, or proteins. In most cases, proteins are long, linear polymers of amino acids that are connected to each other via peptide bonds- in the first step of amino acid analysis, these peptide bonds are hydrolyzed. Once the amino acids are separated, they are then detected and quantified.
Amino acid analysis is one of the major analytical techniques used in biochemical or biotechnological environments. It can be used to answer a range of biological questions. It is the method of choice in order to get reliable protein/peptide quantitation.