BD, 550389, BD Pharmingen™ Purified Mouse Anti-Human CD31

Alternative Name: EndoCAM; GPIIA'; PECA1; PECAM1; Platelet endothelial cell adhesion molecule
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
Workshop Number: V P025
RRID: AB_2252087
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: For optimal indirect immunohistochemical staining, investigators are encouraged to titrate the Mouse Anti-Human CD31 antibody (clone WM59) (suggested starting dilution of 1:10 to 1:50) and visualize staining via a three-step staining procedure in combination with Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) using a DAB detection system (Cat. No. 550880). Alternatively, investigators may want to consider using the Anti-Mouse Ig HRP Detection Kit (Cat.No. 551011), which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.