BD, 550878, BD Pharmingen™ Purified Mouse IgG1 κ Isotype Control

Isotype: Mouse BALB/c IgG1, κ
Application: ELISA, Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development)
Concentration: 250 µg/ml
RRID: AB_10052281
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures This antibody has been tested by ELISA and Flow cytometry to assure specificity and reactivity. For immunohistochemical staining of acetone-fixed frozen sections or formalin-fixed paraffin sections, a dilution range of 1:12.5 - 1:50 is suggested.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.