BD, 551506, BD Pharmingen™ Biotin Anti-Mouse IFN-γ

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Partially-Purified Mouse IFN-γ
Application: ELISA Detection (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394224
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Detection: The R4-6A2 antibody has been found to be useful in sandwich ELISAs that measure mouse IFN-γ protein levels. The Biotin Anti-Mouse IFN-γ antibody (Cat. No. 551506) can be used as a detection antibody along with Purified Rat Anti-Mouse IFN-γ (Cat. No. 551309) as the capture antibody and Recombinant Mouse IFN-γ (Cat. No. 554587) as the standard. The Biotin Anti-Mouse IFN-γ antibody should be titrated 0.5 -2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of mouse IFN-γ ranging from ~500 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT."  For maximal sensitivity, an overnight incubation (4°C) of samples/standards with the coated capture antibody is suggested. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IFN-γ in serum or plasma our Mouse IFN- γ (AN-18) ELISA Set (Cat. No. 551866) or Mouse IFN- γ ELISA Kit II (Cat. No. 558258) are specially formulated and recommended.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.