BD, 554398, BD Pharmingen™ Purified NA/LE Rat Anti-Mouse IL-6

Alternative Name: Il6; Il-6; Interleukin-6; B-cell hybridoma growth factor
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1
Immunogen: Mouse IL-6 Recombinant Protein
Application: ELISA Capture (Routinely Tested), Neutralization (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_2127480
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA :  Purified Rat Anti-Mouse IL-6 antibody (Cat. No. 554400 or 554398) can be useful as a capture antibody for sandwich ELISA measuring mouse IL-6 protein levels.  Purified Rat Anti-Mouse IL-6 antibody can be paired with Biotin Rat Anti-Mouse IL-6 antibody (Cat. No. 554402, clone MP5-32C11) as the detecting antibody, with recombinant mouse IL-6 (Cat. No. 554582) as the standard. For testing mouse IL-6 in complex biological samples, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-6 ELISA Set (Cat. No 555240) or BD OptEIA™ Mouse IL-6 ELISA Kit (Cat. No. 550950).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.