BD, 554482, BD Pharmingen™ Purified Rat Anti-Human IL-4

Reactivity: Human (QC Testing)
Isotype: Rat IgG1
Immunogen: Purified Recombinant Human IL-4
Application: Intracellular block/flow cytometry (Routinely Tested), Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_398561
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: 1. Blocking Control for Intracellular Staining: The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody (Cat. No. 554486, 554485, No. 554484). To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (Cat. No. 554482) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody.  The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. 2. Neutralization: The NA/LE™ MP4-25D2 antibody (Cat. No. 554481) is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody, Cat. No. 554682. 3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.