BD, 554522, BD Pharmingen™ PE Mouse Anti-Human CD122

Alternative Name: IL-2 Receptor β chain; IL2RB; IL-2Rβ; IL15RB; P70-75
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Application: Flow cytometry (Routinely Tested), Blocking (Reported)
Concentration: 0.2 mg/ml
Workshop Number: V C045
RRID: AB_395451
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures The antibody from clone Mik-β2 has been reported to inhibit dose-dependently the [125]I-IL-2 binding to YTS cells, which express the β chain alone, with a 50% binding inhibition at 3 mg/ml. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.