BD, 554686, BD Pharmingen™ APC Rat IgG1, κ Isotype Control

Isotype: Rat IgG1, κ
Immunogen: Mouse immunoglobulin
Application: Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398577
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining for Intracellular Cytokines: The APC-R3-34 immunoglobulin (Cat. No. 554686) is a suitable rat IgG1, κ isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse or human cells for flow cytometric analysis. Use at comparable concentrations to antibody of interest. For specific methodology, please visit our website, www.bdbiosciences.com and go to the protocols section or the see Chapter 4: Immunofluorescent Staining of Intracellular Molecules for Flow Cytometric Analysis. Techniques for Immune Function Analysis Application Handbook 1st Edition. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.