BD, 555003, BD Pharmingen™ Purified Hamster Anti-Rat CD29

Alternative Name: Itgb1; Integrin β1 chain; Integrin beta-1; VLA-4 subunit beta
Reactivity: Rat (QC Testing), Mouse (Tested in Development)
Isotype: Armenian Hamster IgM, κ
Immunogen: Rat glomerular epithelial cells
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395637
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Preliminary studies have shown that azide-containing antibody preparations non-specifically interfere with some in vitro adhesion assays. Therefore, we recommend the No Azide/Low Endotoxin (NA/LE™) format of the Ha2/5 antibody and NA/LE™ hamster IgM isotype control (Cat. No. 553957) for in vitro blocking studies. We recommend our immunohistochemistry formulation of purified anti-mouse CD29 mAb HMβ1-1, Cat. No. 550530, for immunohistochemical staining (IHC) of rat tissues. For IHC of mouse tissues, we recommend the use of purified anti-mouse CD29 mAb 9EG7 in our special formulation for immunohistochemistry, Cat. No. 550531.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.