BD, 556960, BD Pharmingen™ Purified Rat Anti-Mouse IgA

Alternative Name: IgA; Igh-2; Igha; Immunoglobulin heavy constant alpha
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: Pooled mouse IgA
Application: ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_2075740
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich mouse, Biotin Rat Anti-Mouse IgA (Cat. No. 556978) is optimal for detection with Purified Rat Anti-Mouse IgA (Cat. No. 556969) for capture.  Purified Rat Anti-Mouse IgA (Cat. No. 556960) may be used as a primary reagent in immunofluorescent staining and immunohistochemical staining of acetone-fixed frozen sections.  For flow cytometric detection of intracytoplasmic IgA, we recommend FITC Rat Anti-Mouse IgA (Cat. No. 559354).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.