BD, 559045, BD Pharmingen™ Purified Mouse Anti-Human CD15

Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgM, κ
Immunogen: U-937 histiocytic cell line
Application: Flow cytometry (Routinely Tested), Dot Blot, ELISA, Functional assay, Immunohistochemistry-formalin (antigen retrieval required) (Reported)
Concentration: 1.0 mg/ml
RRID: AB_397181
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Functional Studies: Anti-Lewis[x] monoclonal antibody (clone MMA) has been used to determine Le[x] expression on normal and neoplastic cells in studies of tumor metastasis, together with sialyl-Le[x] (CD15s), E-selectin (CD62E) and Pselectin (CD62P) monoclonal antibodies. Immunohistology: Anti-Lewis[x] monoclonal antibody (clone MMA) can be used in staining formalin-fixed paraffin-embedded tissue sections of normal prostate, colon, stomach, brain and lymphoid tissues, adenocarcinomas of lung and urinary bladder. The MMA monoclonal antibody also stains neuraminidase-treated, formalin-fixed, paraffin-embedded sections of normal, metaplastic and neoplastic cervical epithelium. Immunoblotting: Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] in membranous glycoprotein cell or tissue extracts by immunoblotting. Cell ELISA: Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] on HL-60 promyelocytic, HT-29 colon cancer or F9 teratocarcinoma cells.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.