BD, 560155, BD™ Cytometric Bead Array (CBA) Human Soluble TNFRII Flex Set

Assay Range: 10-2,500 pg/mL
Reactivity: Human (QC Testing)
Regulatory Status: RUO
RRID: AB_2869316
Description: Description The BD™ CBA Human TNFRII Flex Set is a bead-based immunoassay capable of measuring soluble human type 2 tumor necrosis factor receptor (TNFRII), also known as CD120b, in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human TNFRII Flex Set. The biology and function of TNFRII has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).
Preparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures: Recommended Assay Procedures The BD CBA Human TNFRII Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264/558265), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human TNFRII Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1. The BD CBA Human TNFRII Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Receptor or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Protein Flex Set reagents . Performance Limit of Detection: The theoretical limit of detection is 1.4 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n =30) + 2 standard deviations.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. ProClin is a trademark of Rohm and Haas Company. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Description : Quantity/Size : Part Number : EntrezGene ID
N/A : 100.0 : N/A : N/A