Bio-Rad, 1632087, ReadyPrep™ Protein Extraction Kit (Signal), 50 preps

50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue
Overview
Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4°C in the presence of octylphenol ethoxylate to isolate proteins associated with lipid rafts.
• Isolate proteins involved in intracellular membrane trafficking and signaling pathways
• After fractionation, proteins are easily solubilized in the supplied buffer
• Solubilized proteins can be used immediately in IEF, SDS-PAGE and other applications
Many proteins located within lipid rafts are resistant to solubilization at 4°C by octylphenol ethoxylate and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.
Proteins that can be isolated using this kit include
• Gycosylphosphatidylinositol (GPI)-anchored proteins
• Caveolin and other caveolae-associated proteins
• Acylated tyrosine kinases
• Heterotrimeric G-proteins
• Some proteins with transmembrane domains
Kit Components
For 50 extractions of 25–50 mg of cells or tissue:
• Signal protein extraction buffer 1 (S1), 50 ml
• Signal protein extraction buffer 2 (S2), 50 ml
• Protein solubilization buffer (PSB), 25 g
• PSB diluent, 30 ml
• Instruction manual
Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.
Related Products
• ReadyPrep Kits for Fractionation by Solubility
• Aurum™ Ion Exchange Mini Kits for Fractionation by Charge
• Rotofor ® Liquid-Phase IEF Cells for Fractionation by pI
• Model 491 Prep Cell and Mini Prep Cell for Fractionation by Solubility
• ReadyPrep™ 2-D Starter Kit
• PROTEAN ® i12™ IEF System
• ReadyStrip™ IPG Strips
• ReadyPrep Reduction-Alkylation Kit ( 1632090 )
• PowerPac™ HV High-Voltage Power Supply
Related Categories
• Protein Gels
• Electrophoresis Chambers
• 2-D Electrophoresis Buffers and Reagents
• Ampholytes
• Tube Gel IEF Systems
• Gel Imaging Systems
• 2-D Electrophoresis Workflow
Supporting Documentation
• ReadyPrep Protein Extraction Kit (Signal) Instruction Manual
• Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure
• ReadyStrip IPG Strip Instruction Manual
• PROTEAN i12 IEF System Brochure
• Host Cell Protein (HCP) Workflow Quick Guide