Qiagen, 333051, QIAseq UPX 3' Targeted 96 index A (384)

Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed)

Features

- Target up to 1000 genes using a cost-effective, time-saving single-tube library prep
- LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
- UMIs eliminate library amplification bias for accurate gene expression
- Cell tagging and sample indexing enables simultaneous sequencing of up to 147,456 targeted libraries
- Includes cloud-based read alignment and single-cell or low-input analysis

Product Details

QIAseq UPX 3' Targeted RNA Panels use unique molecular index (UMIs) and ultraplex (UPX) technology to enable accurate gene expression using 3' RNA-seq from single cells (1–1000), cell pellets and previously isolated total RNA (1 pg to 10 ng). Use our custom builder to design your custom panel for up to 1000 genes. UPX technology allows up to 384 plates of 384 samples (147,456 total samples) to be multiplexed together at one time. QIAseq UPX 3' Targeted Panels are available for 96 or 384 samples. For processing less than 96 samples at a time, use QIAseq UPX 3' Targeted RNA Panel (96) (cat. no. 333041), which is supplied with a breakable plate enabling processing of as few as 24 samples at one time for sequencing. The QIAseq UPX 3' Targeted RNA Panel (96-M) (cat. no. 333042) is for processing 96 samples in parallel 4 times (384 samples). The QIAseq UPX 3' Targeted RNA Panel (384) (cat. no. 333043) is for processing 384 samples simultaneously. QIAseq UPX 3' Targeted RNA Panels are only compatible with QIAseq UPX Targeted RNA Indices, which must be used during library construction for correct indexing.

Performance

Strong reproducibility at ultra-low input RNA amounts

Strong linearity from 10 pg to 1 ng

3' RNA-seq from single cells

Procedure

Innovative, optimized workflow

Applications

- High-throughput screening applications (e.g., toxicology research)
- Single-cell analysis
- Differential gene expression analysis