Waters, 186009495, ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 x 150 mm, 1/pk

The ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm Peptide Separation Technology Columns have been specially optimized and QC tested with cytochrome c digest standards (p/n: 186006371) for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for especially large peptides in proteomics, protein characterization, and peptide synthesis. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Temperature Limits: 80 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 300 Å
QC Tested: Peptide
Format: Premier Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 18 %
eCord: Yes
UNSPSC: 41115711
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 x 150 mm, 1/pk Prioritize speed and resolution in peptide separations with the ACQUITY Premier Peptide BEH C18 Column. Thanks to the Ethylene-Bridged Hybrid (BEH) particle technology in this silica column, you can perform size-exclusionary or reverse-phase chromatographic separations and get terrific peak shape delivered right back. And because data reproducibility is of the utmost importance when you’re hard at work in the lab, this column provides ultra-low bleed so you can remain confident in your data. What’s more, the ACQUITY Premier Peptide BEH C18 Column has earned the MaxPeak designation, which means this UPLC column employs breakthrough technology to reduce unwanted analyte-surface interactions. Proteomics by nature presents a series of obstacles that even the most experienced scientists will have to overcome. That’s why the ACQUITY Premier Peptide BEH C18 Column was designed specifically for those assays and others involving peptide separations, including protein characterization. But even more than that, this UPLC column provides outstanding mechanical stability at increased temperatures needed for peptide mapping, and it offers a wide pH range to accommodate various analytes. Add in the MaxPeak High-Performance Surface technology and it’s easy to see why so many scientists turn to this product for protein analysis. Need to use TFA-containing eluents? Thanks to the low-positive-charged surface contained in this column, you can do exactly that. But that’s not the only flexibilities offered by the ACQUITY Premier Peptide BEH C18 Column, as this UPLC tool provides incredible chemical stability. Couple that with the small pore size and increased resolution compared to other columns and it becomes immediately apparent why ACQUITY Premier Columns exist at the forefront of the market. Even if you’re not performing proteomics-related assays, you’ll want the speed and increased resolution offered by ACQUITY Premier Columns. With 8 different UPLC particles of various substrate technologies available, you’re sure to find a silica column that’s right for your application. Shop ACQUITY Columns to find the right match, or tell your lab manager today that you want an ACQUITY Column at your disposal. Don’t just pave a path forward to MS adoption, stock your lab with all of the incredible equipment offered by Waters, including quality control reference materials, lab informatics and automation solutions. Shop for lab equipment now to explore everything we have to offer.

FAQ
What Is A Sample Matrix? A sample matrix is used to describe everything that is present in the typical sample except for the analytes of interest.