Agilent, 5064-8259, ZORBAX 300SB-C18 3.5µm, 0.3 x 100 mm

ZORBAX 300SB-C18 3.5µm, 0.3 x 100 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.3 mm
LC Platform: Stainless Steel
Length: 100 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The ZORBAX 300SB-C18 column is engineered for high-resolution biomolecule separations, featuring a 3.5 micron particle size and a 0.3 x 100 mm dimension ideal for advanced microbore HPLC and UHPLC systems. This reversed-phase column provides outstanding performance for peptide mapping, protein digests, and analysis of small proteins, thanks to its stable-bond C18 chemistry and 300 angstrom pore size that accommodate larger biomolecules with reliable retention and reproducibility.

Optimized for applications in proteomics, biopharmaceutical research, and peptide purity analysis, this column excels in LC-MS workflows requiring low sample volumes and high sensitivity. Its durable silica-based construction allows for high-pressure operation, while endcapping reduces silanol activity, ensuring sharp peak shapes and minimal analyte tailing.

Compatible with Agilent 1100, 1200, 1260, and 1290 Infinity liquid chromatography systems as well as other major HPLC and UHPLC instruments that support microbore columns, this product is also complemented by a suite of ZORBAX guard columns and sample preparation accessories. Its robust performance and versatile design make it an excellent choice for demanding analytical laboratories seeking reproducible results and long column life in peptide and protein analysis.