Agilent, 5065-9910, ZORBAX 300SB-C18, 3.5 µm, 0.10 x 150 mm

ZORBAX 300SB-C18, 3.5 µm, 0.10 x 150 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.1 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The Agilent ZORBAX 300SB-C18 column, featuring 3.5 micron particle size and dimensions of 0.10 x 150 millimeters, is engineered for high-performance reversed-phase liquid chromatography. This narrow-bore column is designed for the analysis of proteins, peptides, and other biomolecules, providing stable and reproducible retention under low to mid-pH conditions. Its StableBond C18 chemistry ensures enhanced column lifetime, even in the presence of trifluoroacetic acid or other strong acidic modifiers, making it ideal for demanding proteomics workflows.

Typical usage scenarios include separation and quantification of complex biological samples in pharmaceutical, biotechnology, and academic research laboratories. The column's small internal diameter supports high-sensitivity applications and compatibility with nano-LC and capillary LC systems, significantly reducing sample and solvent consumption while maintaining robust peak resolution.

The column is fully compatible with Agilent 1100, 1200, 1260 Infinity, and 1290 Infinity LC systems, as well as a wide range of nano-LC and capillary LC instrumentation. It can be utilized with Agilent ZORBAX guard columns and integrated in workflows involving LC-MS for protein characterization, peptide mapping, and biomarker discovery, ensuring high reproducibility and sensitivity for advanced bioanalytical methods.