Agilent, 5190-2503, Agilent Bio SEC-3, 100Å, 4.6 x 300 mm, 3 µm

Agilent Bio SEC-3, 100Å, 4.6 x 300 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3, 100 Angstrom, 4.6 x 300 mm, 3 micron column is designed for robust and precise size exclusion chromatography (SEC) applications in protein and biomolecule analysis. Featuring rigid silica-based particles with a 100 Angstrom pore size and 3 micron particle diameter, this column delivers high-resolution separation of low molecular weight biomolecules, peptides, and small proteins. Its dimensions ensure compatibility with standard HPLC systems and allow for efficient sample throughput without compromising resolution. Ideal for research and quality control laboratories, the column supports detailed molecular weight characterization, aggregate quantification, and purity assessments. It is suitable for biopharmaceutical development, academic research, and quality assurance in protein therapeutics. This column is fully compatible with Agilent 1100, 1200, and 1260 Infinity LC systems, as well as other conventional HPLC platforms. For method optimization, it can be paired with additional Agilent Bio SEC-3 columns of different pore sizes or particle diameters, providing enhanced flexibility for various separations. Offering exceptional lot-to-lot consistency, the column ensures reproducible results and reliable performance in demanding laboratory workflows. It is recommended for use with aqueous mobile phases, under standard SEC operating conditions, and is supported by comprehensive technical documentation for streamlined method development.