Agilent, 5190-2532, Agilent Bio SEC-5, 500Å, 7.8 x 150 mm, 5 µm

Agilent Bio SEC-5, 500Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 15000 Da
Molecular Weight Upper Limit: 5000000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 500 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with a 500 Angstrom pore size and dimensions of 7.8 x 150 mm is engineered for high-resolution size exclusion chromatography of biomolecules, including proteins, peptides, and nucleic acids. Featuring robust, uniformly sized 5 micron silica particles and optimal pore structure, this column delivers precise molecular weight separation and exceptional reproducibility. It is especially suitable for the analysis of monoclonal antibodies, protein aggregates, and other biomacromolecules in both research and quality control environments.

This column supports a wide range of aqueous mobile phases, making it ideal for biomolecule characterization under native conditions. Typical applications include protein purity assessment, aggregation studies, and molecular sizing for pharmaceutical development and academic research. Compatible with standard HPLC and UHPLC systems, it integrates seamlessly with Agilent's bio-inert LC instrumentation as well as other leading chromatographic platforms. For extended workflow flexibility, this column can be paired with other pore size variants from the Bio SEC-5 series or used in conjunction with Agilent's advance sample preparation products. Engineered for repeatable performance and long column lifetime, it is the preferred choice for reliable and efficient SEC analyses in demanding laboratory settings.